What's Up, Doc?
I spent so much time with this group when I was younger that this was surprisingly easy to populate. Most difficult choice was who to use for T (sorry Taz).
Have fun figuring out who is who. I'll give you one: X is Ex (who debuted with 1935's I Haven't Got a Hat).
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EDIT: Depicted are A. Flea, Bugs Bunny, Cecil Turtle, Daffy Duck, Elmer Fudd, Foghorn Leghorn, Granny, Witch Hazel, Instant Martian, Junyer Bear, K-9, Pepe Le Pew, Marvin the Martian, Nasty Canasta, Owl Johnson, Porky Pig, Quentin Quail, Road Runner, Sylvester, Tweety Bird, Uncle Sam, Senor Vulturo, Wile E. Coyote, Ex, Yosemite Sam, Baby Zebra